Descriptive Chart

Here are my final results!

MORPHOLOGICAL CHARACTERISTICS

Cell shape: Bacilli
Spores: No endospores
Gram's Stain: Gram negative
Motility: Not motile
Capsules: None
Special Stains: Non-acid fast

CULTURAL CHARACTERISTICS

Colonies
     Nutrient Agar: Did not do
     Blood Agar: Did not do
Agar Slant: White colony
Nutrient Broth: Growth throughout broth
Oxygen Requirements: Facultative
Optimum Temp: 37 degree's C

PHYSIOLOGICAL CHARACTERISTICS

	TEST	RESULTS
FERMENTATION	Glucose	Acid / Gas
	Lactose	Acid/ Gas
	Sucrose	No Fermentation
	Mannitol	Non-Fermenter
HYDROLYSIS	Gelatin Liquefaction	Negative
	Starch	Positive
	Casein	Negative
	Fat	Negative
IMVIC	Indole	Positive
	Methyl Red	Positive
	Voges-Proskauer	Negative
	Citrate Utilization	Negative
	Nitrate Reduction	Positive
	Urease	Negative
	Catalase	Negative (very weak bubbling)
	Oxidase	Negative

Because my bacteria is a Gram negative, Bacilli shaped, Lactose positive, Indole positive, and Citrate negative bacteria, My bacteria is.......E. Coli!

Nov 22

We did a an exercise studying the technique of immunology and we applied it in a test for food purity. Today I worked with Katherine.

What is immunology? = the Study of an organism's response to a foreign organic substance.

What we did
Took a dropping pipet and a petri dish containing agar. The bottom of each dish was labeled I, II, or III.

Placed the petri dish over the appropriate template so the template and section matched.
Held the dropping pipet bertically over one of the circles on teh template. Squeezing the pipet bulb we gently touched the pipet tip to the surface of the agar. While relasing the bulb we pushed the pipet tip down through the agar to the bottom of the dish, and lifted the pipet vertically. This left a straight- walled well in the agar.

Then, following the instructions, I filled each well.

EXERCISE 1 - Diffusion and Precipitation in Agar

Using template I, I made four wells and then filled them.

well 1 = Green Dye
well 2 = Red Dye
well 3 = Barium Chloride
well 4 = Potassium Sulfate

Closer look at wells and dye

Adding the dyes

Results

EXERCISE 2 - Anitbody-antigen Reaction in Agar

Placed petri dish section II over template II, made the wells as shown and filled them.

well 1= Bovine Albumin

well 2 = Goat Anti-horse Albumin
well 3 = Goat Anti-bovine Albumin
well 4 = Goat Anti-swine Albumin

 

Results for II. Notice the small almost unnoticeable streak that looks like an apostrophe between the M and S. That small mark  shows that there was a reaction.

 The reaction took place between the Bovine Albumin and the Goat Anti-Horse Albumin.


EXERCISE 3 - Testing For Food Purity

Placed the petri dish section III over template III, made the wells as shown and filled them.

well 1= Hamburger Extract
well 2 = Goat Anti-horse Albumin
well 3 = Goat Anti-bovine Albumin
well 4 = Goat Anti-swine Albumin

This reaction took place right under the K between the Hamburger extract and the Goat Anti-Horse Albumin.

Total results