Friday, October 7, 2011

Sept 8

PETRI DISH LAB EXPERIMENT


 Pictured above is our Petri dish for our sample taken from the bottom of a shoe (Rain Boot). The Petri dish shown illustrates separate bacteria colonies, after two days in the incubator. Below are 3 images taken from the same sample under a microscope at various levels of magnification. Notice the different colors of the separate colonies.




Color: Cream & yellow
Elevation: Raised, Crateriform, flat, convex, hilly
Form: Circular and irregular
Margin: Undulate
Appearance: mostly Opaque and very little translucent

Our objective was to isolate and create a pure culture of a single bacterial colony by using the Streak Plate Method. After we examined and documented what kinds of colonies we had in our Petri dishes, we sterilized an inoculating loop, picked a single colony from our Petri dish using the loop and transferred the colony onto another Petri dish that contained an agar medium. Using the loop we spread the colony onto the agar into four quadrants. We placed the new Petri dish into the incubator to be examined next week. The original Petri dish was placed in the refrigerator.  

Conclusion: Our lab experiment demonstrated the growth and quality of the various bacteria found from our sample. It is perhaps surprising to learn how quickly bacteria can grow, and all the places of which it can be found.

Don Caldwell
Melanie Seitz

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